N-hydroxysuccinimide Dcm, Edc
نویسنده
چکیده
Additional Acknowledgements Magnetic resonance imaging work was performed at the Northwestern University Center for Advanced Molecular Imaging generously supported by NCI CCSG P30 CA060553 awarded to the Robert H Lurie Comprehensive Cancer Center. Metal analysis was performed at the Northwestern University Quantitative Bioelemental Imaging Center generously supported by NASA Ames Research Center Grant NNA04CC36G. Imaging work was performed at the Northwestern University Biological Imaging Facility generously supported by the NU Office for Research. Electron microscopy was performed on a JEOL 3200 FETEM purchased with the support of NCRR 1S10RR025092. This work was supported by the Northwestern University Flow Cytometry Facility and a Cancer Center Support Grant (NCI CA060553).
منابع مشابه
Comparison of 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide Based Strategies to Crosslink Antibodies on Amine-Functionalized Platforms for Immunodiagnostic Applications
1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) alone, and in combination with N-hydroxysuccinimide (NHS) or sulfoNHS were employed for crosslinking anti-human fetuin A (HFA) antibodies on 3-aminopropyltriethoxysilane (APTES)-functionalized surface plasmon resonance (SPR) gold chip and 96-well microtiter plate. The SPR immunoassay and sandwich enzyme linked immunosorbent immunoassay (ELISA...
متن کاملSupporting Informations Human serum biomarker detection based on cascade signal amplification strategy by DNA molecule machine
Reagents. Thioglycolic acid (TGA), Bovine serum albumin (BSA), imidazole, N-(3dimethylaminopropyl)-N’-ethyl-carbodiimide hydrochloride (EDC), Nhydroxysuccinimide (NHS), (+)-Biotin N-hydroxysuccinimide were purchased from Sigma-Aldrich. EDC and NHS were dissolved in water immediately before use. Human prostate-specific antigen (PSA), two mouse antihuman total PSA monoclonal antibodies (primary c...
متن کاملCorneal Stromal Cell Growth on Gelatin/Chondroitin Sulfate Scaffolds Modified at Different NHS/EDC Molar Ratios
A nanoscale modification strategy that can incorporate chondroitin sulfate (CS) into the cross-linked porous gelatin materials has previously been proposed to give superior performance for designed corneal keratocyte scaffolds. The purpose of this work was to further investigate the influence of carbodiimide chemistry on the characteristics and biofunctionalities of gelatin/CS scaffolds treated...
متن کاملEffect of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide concentrations on the mechanical and biological characteristics of cross-linked collagen fibres for tendon repair.
Reconstituted type I collagen fibres have received considerable interest as tendon implant materials due to their chemical and structural similarity to the native tissue. Fibres produced through a semi-continuous extrusion process were cross-linked with different concentrations of the zero-length cross-linker 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) in combination with N-hydroxysucc...
متن کاملCross-linking of dermal sheep collagen using a water-soluble carbodiimide.
A cross-linking method for collagen-based biomaterials was developed using the water-soluble carbodiimide 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide hydrochloride (EDC). Cross-linking using EDC involves the activation of carboxylic acid groups to give O-acylisourea groups, which form cross-links after reaction with free amine groups. Treatment of dermal sheep collagen (DSC) with EDC (E-DSC)...
متن کامل